Susan’s work centres on utilising X-ray Micro-Computed Tomography to visualise plants growing in soil and is part of an interdisciplinary project with the School of Computer Science.
The project aims to isolate root architecture traits that improve phosphorus use efficiency in crop species. In general, shallower root systems are more successful at acquiring phosphorus. The overarching hypothesis for this work is that agravitropic root systems of model plants Arabidopsis thaliana and Oryza sativa will more efficiently extract phosphorus from soil than their wildtype, gravitropic counterparts. Most experiments that pinpoint gene expression and signalling related to phosphorus deficiency employ non-soil media such as agar or sand. This is likely because of the heterogeneity of soil and the difficulty for visualisation in the opaque medium. X-ray Micro-Computed Tomography (CT) will be used to non-destructively image 3D root architecture and microscale soil characteristics over time.
Published Work
- Stefan Mairhofer, Susan Zappala , Saoirse Tracy, Craig J. Sturrock, Malcolm Bennett, Sacha J. Mooney, Tony Pridmore (2013) Recovering complete plant root system architectures from soil via X-ray μ-Computed Tomography. Plant Methods 9:8
Root Systems Architecture
- Susan Zappala , Stefan Mairhofer, Saoirse Tracy, Craig J. Sturrock, Malcolm Bennett, Tony Pridmore, Sacha J. Mooney (2013) Quantifying the effect of soil moisture content on segmenting root system architecture in X-ray computed tomography images. Plant and Soil
Root Systems Architecture
- Mairhofer S, Zappala S, Tracy S, Sturrock C, Bennett M, Mooney S, Pridmore T (2011) RοοTrak: Automated Recovery of Three-Dimensional Plant Root Architecture in Soil from X-Ray Microcomputed Tomography Images Using Visual Tracking. Plant Physiology 158, 561–569
Root Systems ArchitectureVirtual Root
- Lucas M, Swarup R, Paponov IA, Swarup K, Casimiro I, Lake D, Péret B, Zappala S, Mairhofer S, Whitworth M, Wang J, Ljung K, Marchant A, Sandberg G, Holdsworth MJ, Palme K, Pridmore T, Mooney S, Bennett MJ (2010) Short-Root regulates primary, lateral, and adventitious root development in Arabidopsis. Plant Physiology 155, 384–98
Virtual Root