Localisome

Information about our recombineering and other localisome resources

Recombineering resources have been generated in partnership with the lab of Jose Alonso.

Large Arabidopsis artificial chromosomes can be precisely tagged with fluorescence proteins by homologous recombination in E. coli and then transferred to the Arabidopsis genome. The system is described in a paper available here. A Venus or YPET translation fusion was generated by recombineering and the Venus yellow fluorescent protein DNA sequence (obtained from Dr. Oliver Hobert at Columbia University Medical Center, New York published in PLOS One) was typically fused in frame with the target gene sequence just before the stop codon.

More information available here.